日下部研究室

A. 学術論文情報（Papers）

1. Production of an active Mus musculus IL-3 using updated silkworm-based baculovirus expression vector system.

Nagai Ryo, Ebihara Takeru, Kakino Kohei, Masuda Akitsu, Xu Jian, Minamihata Kosuke, Kamiya Noriho, Kongkrongtong Tatphon, Kawahara Masahiro, Mon Hiroaki, Tsuguru Fujii, Takahiro Kusakabe, Jae Man Lee,
Journal of Asia-Pacific Entomology, 2021, 24, 3, 544-549.
https://www.sciencedirect.com/science/article/pii/S1226861521000558?via%3Dihub

2. Venestatin from parasitic helminths interferes with receptor for advanced glycation end products (RAGE)-mediated immune responses to promote larval migration.

Tsubokawa Daigo, Kikuchi Taisei, Lee Jae Man, Kusakabe Takahiro, Yamamoto Yasuhiko, Maruyama Haruhiko,
PLoS pathogens,2021, 17, 6, e1009649.
https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1009649

3. Active Human and Murine Tumor Necrosis Factor α Cytokines Produced from Silkworm Baculovirus Expression System.

Ebihara Takeru, Xu Jian, Tonooka Yoshino, Nagasato Takumi, Kakino Kohei, Masuda Akitsu, Minamihata Kosuke, Kamiya Noriho, Nakatake Hirokazu, Chieda Yuuka, Hiroaki Mon, Tsuguru Fujii, Takahiro Kusakabe, Jae Man Lee,
Insects, 2021, 12, 6, 517
https://www.mdpi.com/2075-4450/12/6/517

4. Stable trimer formation of spike protein from porcine epidemic diarrhea virus improves the efficiency of secretory production in silkworms and induces neutralizing antibodies in mice.

Masuda Akitsu, Lee Jae Man, Miyata Takeshi, Ebihara Takeru, Kakino Kohei, Hino Masato, Fujita Ryosuke, Mon Hiroaki, Kusakabe Takahiro, 
Veterinary Research, 2021, 52, 1, 1-14.
https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-021-00971-5

5. Production of scFv, Fab, and IgG of CR3022 Antibodies Against SARS-CoV-2 Using Silkworm-Baculovirus Expression System.

Ebihara Takeru, Masuda Akitsu, Takahashi Daisuke, Hino Masato, Mon Hiroaki, Kakino Kohei, Fujii Tsuguru, Fujita Ryosuke, Ueda Tadashi, Lee Jae Man, Takahiro Kusakabe,
Molecular Biotechnology, 2021, 63,12, 1223-1234
https://link.springer.com/article/10.1007/s12033-021-00373-0

6. Non-molting dwarf (nm-d) as a mutant of Bombyx mori with a defect in purine synthesis.

Fujii Tsuguru, Kakino Kohei, Fukumori Hisayoshi, Hino Masato, Lee Jae Man, Kusakabe Takahiro, Banno Yutaka, 
Insect Biochemistry and Molecular Biology, 2021, 138, 103636.
https://www.sciencedirect.com/science/article/pii/S0965174821001193

7. Leptin Is an Important Endocrine Player That Directly Activates Gonadotropic Cells in Teleost Fish, Chub Mackerel.

Ohga Hirofumi, Ito Kosuke, Kakino Kohei, Mon Hiroaki, Kusakabe Takahiro, Lee Jae Man, Matsuyama Michiya, 
Cells, 2021, 10, 12, 3505.
https://www.mdpi.com/2073-4409/10/12/3505

8. Optimization of SARS-CoV-2 Spike Protein Expression in the Silkworm and Induction of Efficient Protective Immunity by Inoculation With Alum Adjuvants.

Masuda Akitsu, Lee Jae Man, Miyata Takeshi, Mon Hiroaki, Sato Keita, Oyama Kosuke, Sakurai Yasuteru, Yasuda Jiro, Takahashi Daisuke, Ueda Tadashi, Yuri Kato, Motohiro Nishida, Noriko Karasaki, Kohei Kakino, Takeru Ebihara, Takumi Nagasato, Masato Hino, Ayaka Nakashima, Kengo Suzuki, Yoshino Tonooka, Miyu Tanaka, Takato Moriyama, Hirokazu Nakatake, Ryosuke Fujita, Takahiro Kusakabe,
Frontiers in Immunology, 2021, 12.
https://www.frontiersin.org/articles/10.3389/fimmu.2021.803647/full

9. Silkworm FoxL21 plays important roles as a regulator of ovarian development in both oogenesis and ovariole development.

Tanaka Miyu, Fujii Tsuguru, Mon Hiroaki, Lee Jae Man, Kakino Kohei, Fukumori Hisayoshi, Ebihara Takeru, Nagasato Takumi, Hino Masato, Tonooka Yoshino, Takato Moriyama, Ryosuke Fujita, Yutaka Banno, Takahiro Kusakabe,
Insect Biochemistry and Molecular Biology, 2022, 143, 103737.
https://www.sciencedirect.com/science/article/pii/S0965174822000194?via%3Dihub

10. Highly efficient protein expression of Plasmodium vivax surface antigen, Pvs25 by silkworm, Bombyx mori, and its biochemical analysis.

Miyata Takeshi, Minamihata Kosuke, Kurihara Koichi, Kamizuru Yui, Gotanda Mari, Obayashi Momoka, Kitagawa Taiki, Sato Keita, Kimura Momoko, Oyama Kosuke, Yuta Ikeda, Yukihiro Tamaki, Jae Man Lee, Kozue Sakao, Daisuke Hamanaka, Takahiro Kusakabe, Mayumi Tachibana, Hisham H Ibrahim;
https://bioRxiv, https://www.biorxiv.org/content/10.1101/2022.03.03.482736v1

B. 総説・解説（Reviews）・著書(Books)

1. Production of antiviral vaccines using a silkworm-baculovirus expression system

Kusakabe Takahiro
Nihon Yakurigaku zasshi. Folia Pharmacologica Japonica, 2022, 157, 2, 128-133.
https://www.jstage.jst.go.jp/article/fpj/157/2/157_21063/_article/-char/ja/

C. 特許情報（Patents）

該当なし

D. その他の活動（プレスリリース・新聞記事等・広報資料）

該当なし